Publications

ReseacherID (A-8536-2008)

Legend: † Shared first or senior authorship; Papers where I am the correspondent author

Submitted
available at bioRxiv or upon request while in peer-review

Campbell CJ, Venkitaraman AR, Esposito A*
Checkpoint non-fidelity induces a complex landscape of lineage fitness after DNA damage
bioRxiv

Fries MW, Haas KT, Ber S, Saganty J, Richardson EK, Venkitaraman AR†, Esposito A,*
Multiplexed biochemical imaging reveals caspase activation patterns underlying single-cell fate
bioRxiv

2021

A Trinh and A Esposito
The biochemical resolving power of fluorescence lifetime imaging: untangling the roles of the instrument response function and photon-statistics

bioRxiv (now accepted in Biomedical Optics Express)

KT Haas, M Fries, AR Venkitaraman, and A Esposito
Single-cell biochemical multiplexing by multi-dimensional phasor DEmixing AND SPECTRALLY RESOLVED FLIM
Frontiers in Physics (accepted)

Esposito A*
Cooperation of partially-transformed clones: an invisible force behind the early stages of carcinogenesis
Royal Society Open Science Journal 8: 201532

Scott DE, Francis-Newton NF, Marsh ME, Coyne AG, Fischer G, Moschetti T, Bayly AR, Sharpe TD, Haas K, Barber L, Valenzano C, Srinivasan R, Huggins DJ, Ehebauer M, Esposito A, Pellegrini L, Perrior T, McKenzie G, Blundell TL, Hyvonen M, Skidmore J, Venkitaraman AR, Abell C
A small-molecule inhibitor of the BRCA2-RAD51 interaction modulates RAD51 assembly and potentiates DNA damage-induced cell death
Cell Chemical Biology 28:1-13

2020

De S, Campbell CJ, Venkitaraman AR†, Esposito A,*
Pulsatile MAPK signalling modulates p53 activity to control cell fate decisions at the G2 checkpoint for DNA damage
Cell Reports 30(7):2083-2093

Esposito A*
How many photons are needed for FRET imaging?
Biomedical Optics Express 11(2):1186-1202

2019

Trinh AL, Ber S, Howitt A, Oriol Valls P, Fries MW, Venkitaraman AR, Esposito A*
Fast single-cell biochemistry: theory, open-source microscopy and applications
Methods Appl. Fluoresc. 7:044001

Esposito A* and Venkitaraman AR
ENHANCING BIOCHEMICAL RESOLUTION BY HYPER-DIMENSIONAL IMAGING MICROSCOPY
Biophys. J. 116(10):1815-1822

2013-2018

Haas KT, Lee M, Esposito A† and Venkitaraman AR†
SINGLE-MOLECULE LOCALIZATION MICROSCOPY REVEALS MOLECULAR TRANSACTIONS DURING RAD51 FILAMENT ASSEMBLY AT CELLULAR DNA DAMAGE SITES
Nucleic Acids Research 46(5):2398–2416

Popleteeva A, Stoppa D, Pancheri L, Gasparini L, Kaminski CF, Cassidy LD, Venkitaraman AR, Esposito A*
FAST AND SIMPLE SPECTRAL FLIM FOR BIOCHEMICAL AND MEDICAL IMAGING
Optics Express 23(18):23511-23525

Liang H, Esposito A, De S, Ber S, Collin P, Surana U, Venkitaraman AR
Homeostatic control of the G2 checkpoint via polo-like kinase 1 engenders non-genetic heterogeneity in its fidelity and timing
Nat Comms. 5, 4048

Esposito A*, Popleteeva M, Venkitaraman AR
Maximizing the biochemical resolving power in fluorescence microscopy
PLOS ONE, 8(10):e77392

2012

Kiwanuka S, Laurila T, Jonathan F, Esposito A, Blomberg von der Geest K, Pancheri L, Stoppa D, Kaminski C
DEVELOPMENT OF BROADBAND CAVITY RING-DOWN SPECTROSCOPY FOR BIOMEDICAL DIAGNOSTICS OF LIQUID ANALYTES
Analytical Chemistry, 84 (13):5489–5493

Esposito A*
Beyond range: innovating fluorescence microscopy
Remote Sens. 4(1):111-119

2011

Fereidouni F, Esposito A, Blab GA, Gerritsen HC
A MODIFIED PHASOR APPROACH FOR ANALYZING TIME-GATED FLUORESCENCE LIFETIME IMAGES
J. Microsc. 244(3):248-258

Esposito A*, Bader AN, Schlachter SC, van den Heuvel DJ, Kaminski Schierle GS, Venkitaraman AR, Kaminski CF, Gerritsen HC
DESIGN AND APPLICATION OF A CONFOCAL MICROSCOPE FOR SPECTRALLY RESOLVED ANISOTROPY IMAGING
Opt. Express, 19(3):2546–2555

Kaminski Schierle GS, Bertoncini CW, Chan FTS, van der Goot AT, Schwedler S, Skepper J, Schlachter S, van Ham T, Esposito A, Kumita JR, Nollen EAA, Dobson CM, Kaminski CF
A FRET SENSOR FOR NON-INVASIVE IMAGING OF AMYLOID FORMATION IN VIVO
ChemPhysChem, 12(3):673-680

Mauritz JMA, Seear R, Esposito A, Kaminski CF, Skepper J, Warley A, Lew VL, Tiffert T
X-RAY MICROANALYSIS INVESTIGATION OF THE STAGE-RELATED CHANGES IN NA, K AND HEMOGLOBIN CONCENTRATION IN PLASMODIUM FALCIPARUM -INFECTED RED BLOOD CELLS
Biophys. J., 100(6):1438-1445

2010

Jeyasekharan AD, Ayoub N, Mahen R, Ries J, Esposito A, Rajendra E, Hattori H, Kulkarni RP, Venkitaraman AR
DNA DAMAGE REGULATES THE MOBILITY OF BRCA2 WITHIN THE NUCLEOPLASM OF LIVING CELLS
Proc. Natl. Acad. Sci. USA, 107(50): 21937–21942

Swietach P, Tiffert T, Mauritz JM, Seear R, Esposito A, Kaminski C, Lew VL, Vaughan-Jones RD
HYDROGEN ION DYNAMICS IN HUMAN RED BLOOD CELLS
J Physiol., 588(24):4995-5014

Mauritz J, Tiffert T, Seear R, Lautenschlaeger F, Esposito A, Lew V, Guck J, Kaminski CF
DETECTION OF PLASMODIUM FALCIPARUM-INFECTED RED BLOOD CELLS BY OPTICAL STRETCHING
J. Biomed. Opt., 15(3):030517

Mauritz J, Esposito A, Tiffert T, Skepper JN, Warley A, Yoon YZ, Cicuta P, Lew VL, Guck JR, Kaminski CF
BIOPHOTONIC TECHNIQUES FOR THE STUDY OF MALARIA-INFECTED RED BLOOD CELLS
Med Biol Eng Comput., 48(10):1055-63

van Ham TJ, Esposito A, Kumita JR, Hsu SD, Kaminski-Schierle GS, Kaminski CF, Dobson CM, Nollen EAA and Bertoncini CW
TOWARDS MULTIPARAMETRIC FLUORESCENT IMAGING OF AMYLOID FORMATION: STUDIES ON A YFP MODEL OF ALPHA-SYNUCLEIN AGGREGATION
J Mol Biol 395(3):627-642

Cheung W, Gill M, Esposito A, Kaminski CF, Courousse N, Chwetzoff S, Trugnan G, Keshavan N, Lever A and Desselberger U
ROTAVIRUSES ASSOCIATE WITH CELLULAR LIPID DROPLET COMPONENTS TO REPLICATE IN VIROPLASMS, AND COMPOUNDS DISRUPTING OR BLOCKING LIPID DROPLETS INHIBIT VIROPLASM FORMATION AND VIRAL REPLICATION
J. Virol. 84(13): 6782-6798

Esposito A*, Choimet JB, Skepper JN, Mauritz JMA, Lew VL, Kaminski CF, Tiffert T
QUANTITATIVE IMAGING OF HUMAN RED BLOOD CELLS INFECTED WITH PLASMODIUM FALCIPARUM
Biophys. J., 99(3):953-960

Cueff A, Dyrda A, Bouyer G, Egée S, Seers R, Esposito A, Tiffert T, Lew VL and Thomas SLY
EFFECTS OF ELEVATED INTRACELLULAR CALCIUM ON THE OSMOTIC FRAGILITY OF HUMAN RED BLOOD CELLS
Cell Calcium, 47(1):29-36

2009

Schlachter S, Schwedler S, Esposito A, Moggridge GD, Kaminski GS and Kaminski CF
A METHOD TO UNMIX MULTIPLE FLUOROPHORES IN MICROSCOPY IMAGES WITH MINIMAL A PRIORI INFORMATION
Opt. Express, 17(25):22747-22760

Wagner OI, Esposito A, Shen K, Wenzel D, Koehler B, Wouters FS and Klopfenstein DR
SYNAPTIC SCAFFOLDING PROTEIN SYD-2 CLUSTERS AND ACTIVATES KINESIN-3 UNC-104 IN C. ELEGANS
Proc. Natl. Acad. Sci. USA, 106(46):19605-10

Mauritz J, Esposito A*, Ginsburg H, Kaminski CF, Tiffert T and Lew VL
THE HOMEOSTASIS OF PLASMODIUM FALCIPARUM INFECTED RED BLOOD CELLS
PLoS Comp Biol 5(4):e1000339.doi:10.1371/journal.pcbi.1000339

Schlachter S, Elder AD, Esposito A, Kaminski GS, Frank GH, van Geest LK, and Kaminski CF
MHFLIM: RESOLUTION OF HETEROGENEOUS FLUORESCENCE DECAYS IN WIDEFIELD LIFETIME MICROSCOPY
Opt Express 17(3):1557-1570

Babuke T, Ruonala M, Meister M, Amaddii M, Genzler C, Esposito A, Tikkanen R
HETERO-OLIGOMERIZATION OF REGGIE-1/FLOTILLIN-2 AND REGGIE-2/FLOTILLIN-1 IS REQUIRED FOR THEIR ENDOCYTOSIS
Cell Signal, 21(8):1287-97

Papusheva E, Mello de Queiroz F, Dalous J, Han Y, Esposito A, Jares-Erijman E, Jovin TM, and Bunt G
DYNAMIC CONFORMATIONAL CHANGES IN THE FERM DOMAIN OF FAK ARE INVOLVED IN FOCAL ADHESION BEHAVIOR DURING CELL SPREADING AND MOTILITY
J Cell Sci 122, 656-666

2008

Elder AD, Domin A, Kaminski Schierle GS, Lindon C, Pine J, Esposito A, and Kaminski CF
A QUANTITATIVE PROTOCOL FOR DYNAMIC MEASUREMENTS OF PROTEIN INTERACTIONS BY FRET-SENSITIZED FLUORESCENCE EMISSION
J R Soc Interface 6(S1):S59-S81

Esposito A* , Tiffert T, Mauritz J, Schlachter S, Bannister LH, Kaminski CF, Lew VL
FRET IMAGING OF HAEMOGLOBIN CONCENTRATION IN PLASMODIUM FALCIPARUM INFECTED ERYTHROCYTES
PLoS ONE, 3(11): e3780. doi:10.1371/journal.pone.0003780

Esposito A* , Gralle M, Dani MAC, Lange D, Wouters FS
PHLAMELEONS: A FAMILY OF FRET-BASED PROTEIN SENSORS FOR QUANTITATIVE PH IMAGING
Biochemistry, 47(49):13115–13126

Wouters FS and Esposito A
QUANTITATIVE ANALYSIS OF FLUORESCENCE LIFETIME IMAGING MADE EASY
HFSP Journal 2(1):7-11

2007

Esposito A*, Gerritsen HC, Wouters FS
OPTIMIZING FREQUENCY-DOMAIN FLUORESCENCE LIFETIME SENSING FOR HIGH THROUGHPUT APPLICATIONS: PHOTON-ECONOMY AND ACQUISITION SPEED
J. Opt. Soc. Am. A 24(10):3261-3273

Esposito A*, Dohm CP, Bähr M and Wouters FS
UNSUPERVISED FLUORESCENCE LIFETIME IMAGING MICROSCOPY FOR HIGH-CONTENT AND HIGH-THROUGHPUT SCREENING
Mol. Cell. Proteomics 6:1446-1454

Esposito A*†, Dohm CP†, Kermer P, Bähr M, Wouters FS
Α-SYNUCLEIN AND ITS DISEASE-RELATED MUTANTS INTERACT DIFFERENTIALLY WITH THE MICROTUBULE PROTEIN TAU AND ASSOCIATE WITH THE ACTIN CYTOSKELETON
Neurobiol. Dis. 26(3): 521-531

2006

Dohm CP, Siedenberg S, Liman J, Esposito A, Wouters FS, Reed JC, Bähr M, and Kermer P
BAX INHIBITOR-1 PROTECTS NEURONS FROM OXYGEN-GLUCOSE DEPRIVATION
J. Mol Neurosci. 29(1):1-8

Esposito A*, Gerritsen HC, Oggier T, Lustenberger F, Wouters FS
INNOVATING LIFETIME MICROSCOPY: A COMPACT AND SIMPLE TOOL FOR THE LIFE SCIENCES, SCREENING AND DIAGNOSTICS
J. Biomed. Opt. 11(3):34016-34024

2005

Esposito A*, Oggier T, Gerritsen HC, Lustenberger F, Wouters FS, “ALL-SOLID-STATE LOCK-IN IMAGING FOR WIDE-FIELD FLUORESCENCE LIFETIME SENSING”. Opt. Express 13(24):9812-9821

Esposito A*, Gerritsen HC, Wouters FS, “FLUORESCENCE LIFETIME HETEROGENEITY RESOLUTION IN THE FREQUENCY DOMAIN BY LIFETIME MOMENTS ANALYSIS”. Biophys. J. 89(6):1-14

Esposito A*, Cupello A, Pellistri F, Marchetti C, Robello M, “TWO-PHOTON ANALYSIS OF LEAD ACCUMULATION IN CEREBELLAR GRANULE CELLS”. Neurochem. Res. 30(8):949-54

Cupello A, Esposito A, Marchetti C, Pellistri F, Robello M, “CALCIUM ACCUMULATION IN NEURITES AND CELL BODIES OF RAT CEREBELLAR GRANULE CELLS IN CULTURE: EFFECTS ON GABA(A) RECEPTOR FUNCTION”. Amino Acids 28(2):177-182

2004

Pellistri F, Cupello A, Esposito A, Marchetti C, Robello M
TWO-PHOTON IMAGING OF CALCIUM ACCUMULATION IN RAT CEREBELLAR GRANULE CELLS
Neuroreport 15(1):83-87

Esposito A, Federici F, Usai C, Cannone F, Chirico G, Collini M, Diaspro A
NOTES ON THEORY AND EXPERIMENTAL CONDITIONS BEHIND TWO-PHOTON EXCITATION MICROSCOPY
Microsc. Res. Tech.63(1):12-17

Book Chapters

Esposito A*, Schlachter S, Kaminski Schierle GS, Elder AD, Diaspro A, Wouters FS, Kaminski CF, Iliev AI, “QUANTITATIVE FLUORESCENCE MICROSCOPY TECHNIQUES” in Cytoskeleton Methods and Protocols Ed.: Ray H. Gavin, Humana Press Series Methods in Molecular Biology)

Gerritsen HC, Agronskaia S, Bader A, Esposito A (2008), “TIME-DOMAIN FLIM: THEORY AND INSTRUMENTATION” in FRET & FLIM Imaging Techniques, Gadella TWG ed.; Series Laboratory techniques in biochemistry & molecular biology, van der Vliet PC and Pillai S eds.

Esposito A, Gerritsen HC, Wouters FS (2008), “FLUORESCENCE LIFETIME IMAGING MICROSCOPY: QUALITY ASSESSMENT AND STANDARDS”, in Standardization in Fluorometry: State-of-the-Art and Future Challenges Ed.: Resch-Genger U (Springer Series Methods and Applications of Fluorescence, Ed.: Wolfbeis O)

Esposito A, Wouters FS (2004), “FLUORESCENCE LIFETIME IMAGING MICROSCOPY”, in Current Protocols in Cell Biology. Eds.:Bonifacino JS, Dasso M, Harford JB, Lippincott-Schwartz J, and Yamada KM, John Wiley & Sons, New York, NY

Patents

Esposito A, Wouters FS, Lustenberger F, Oggier T, “Apparatus and method for fluorescence lifetime imaging” EP1746410 and US 2007/0018116 A1 (bought by Carl Zeiss GmbH)

Thesis

PhD thesis
MOLECULAR AND CELLULAR QUANTITATIVE MICROSCOPY”, Cuvillier Verlag (Goettingen, Germany) ISBN-10: 3865378293; Prof. H.Gerritsen (supervisor) and Prof. F.Wouters (co-supervisor)

Degree thesis
TWO-PHOTON ANALYSIS OF INTRACELLULAR DIVALENT IONS IN RAT CEREBELLAR GRANULES CULTURE” Prof. M.Robello (supervisor) and Dr. C.Marchetti (co-supervisor)